CB-27 Supplement (50X)

Captivate Bio’s CB-27™ Supplement (50x) is a serum-free solution optimized for the growth and viability of neuronal cells, organoids, and is used with the culture of a variety of cell types. CB-27 is designed for both low- and high-density growth, as well as short- and long-term viability of embryonic neurons. This product is the same as the most widely cited B-27 Supplement for neuronal cell cultures.

CB-27 Supplement (50x) contains a variety of antioxidants designed to mitigate reactive oxidative damage in cells. This product is formulated with water-for-injection, and the recommended working concentration in cell culture media is two percent.

Applications:

• Maintenance/maturation of pre-natal/fetal primary neurons
• Differentiation and maintenance of stem cell-derived neurons
• Proliferation of neural stem cells
• Studies of insulin secretion or insulin receptors
• Maintenance/maturation of stem cell-derived neurons

Keywords: neurons, neuronal cells, organoids, primary neurons, neural stem cells, organoid, spheroids

References

References are publications that support the biological activity of the product.

• Brewer, G.J.; et al. Optimized survival of hippocampal neurons in B27-supplemented Neurobasal, a new serum-free medium combination. Neurosci Res. 1993 Aug 1;35(5):567-76. doi: 10.1002/jnr.4903505
• Brewer, G.J.; et al. Survival and growth of hippocampal neurons in defined medium at low density: advantages of a sandwich culture technique or low oxygen. Brain Res. 1989 Aug 7;494(1):65-74. doi: 10.1016/0006-8993(89)90144-3.
• Strikoudis et al. Cell Rep. 2019;27(12):3709–3723.
• Koning et al. Cell Mol Life Sci. 2020;77(12):2257–73.
• Devall et al. PLoS ONE. 2020;15(1):e0227116.
• Ísberg et al. Sci Rep. 2019; 9:14843.
• Smits et al. NPJ Parkinsons Dis. 2019;5;5 (1)1–8.