CET Cocktail Kits

Maximizing Cell Recovery with Captivate Bio’s CET Cocktail Kits

Whether you're validating a new workflow, supporting cell recovery strategies, or managing high-volume production, Captivate Bio’s CET Cocktail Kits give researchers a smarter way to improve cell survival, recovery, and consistency. Built around the proven CEPT protocol, Captivate Bio’s CET Cocktail Kits are sized to match how you work—by media volume, throughput, and growth stage, all while giving you flexibility along the way. Please note that all kits include Chroman 1, Emricasan, Trans-ISRIB, and Polyamine.

Features:

• Maximum cell survival: Provides comprehensive cytoprotection to overcome cellular stressors inherent in stem cell workflows.
• Translation ready: Individual small molecules can also be available as a custom project in GMP-grade and different pack sizes.
• Scalable: Enables high-volume cell recovery with minimal sourcing challenges.

From validation to core facility demand, choose the variation built for your scale:

CET Spark™ – For early discovery and pilot workflows.
Cat. No. CET02B | 1L
Designed for labs starting new projects or evaluating survival-enhancing supplements, CET Spark™ supports up to 1 liter of media. Ideal for feasibility studies, small teams, and early-stage stem cell programs that need premium performance without overspending.

CET Ignite™ – For routine research and daily culture.
Cat. No. CET01B | 5L
Built for the busiest research labs, CET Ignite™ supports up to 5 liters of media and is the ideal fit for ongoing passaging, thaw recovery, cloning, and regular stem cell maintenance. This is the go-to size for labs that need dependable performance week after week.

CET Titan™ – For core labs and high-volume workflows.
Cat. No. CET03B | 20L
Engineered for scale, CET Titan™ supports up to 20 liters of media for core facilities, translational groups, and high-throughput stem cell programs. When consistency, efficiency, and reduced reorder frequency matter most, Titan delivers.

This combination or variation of small molecules has been shown to directly and efficiently enhance pluripotent stem cell survival and promote clonal growth and expansion of genetically stable hPSCs.¹ The CEPT method allows for safe, controlled, and efficient strategies for the generation, expansion, differentiation, and banking of stem cell-based therapeutic products. This cocktail is also key for optimized embryoid body (EB) and organoid formation, as well as superior cryopreservation of pluripotent and differentiated cells by protecting cells from multiple cellular stress mechanisms in a synergistic fashion.¹

Alternative names: CE(P)T cocktail, CEPT, cet cocktail molecules, CET, CET Spark, CET Ignite, CET Titan
Applications: Stem cell survival, CEPT Cocktail method, cell translation, cryopreservation
Cell types: Pluripotent Stem Cells, iPSCs, ESCs, neurons

References and sample data are from publications that support the biological activity of the product.

Figure 1. Cell survival. hPSCs were seeded as single cells at an ultra-low density of just 25 cells/cm2. Data represents various Y27632, Chroman, Emricassan, and Trans-ISIRB small molecules with a polyamine solution. Improvements are seen in cell survival when using CET and CEPT combinations over Y27632. Chen, Y. et. al. 2021.	Figure 2. Improved clonal growth and expansion of hPSCs. An alkaline phosphatase staining assay of hPSCs cultured with Y27632, the complete CEPT combination, and commercially available CloneR™ supplement, clearly showed more alkaline phosphatase-positive clones in cultures exposed to CEPT. Chen, Y. et. al. 2021.

Keywords: cell recovery, thawing cells, CEPT Cocktail, small molecule, stem cell, embryonic, pluripotent, hESC, hiPSC, hPSC, single-cell passaging, cell dissociation, cell stress, ROCK, ROCK inhibitor, reprogramming, cloning, gene editing, cell survival, cryopreservation, freezing, recovery, attachment, NIH, polyamines, neurons, cardiomyocytes, cell function, cell banking, cell bank, DNA break, cell attachment, cytoprotection, embryoid body, EB, organoid

Documents

• Brochure: CET Cocktail Flyer
• Brochure: Small Molecules for Stem Cell Research
• Research Spotlight: CEPT supplementation demonstrates significant advancement in survival of genetically stable hPSCs
• Research Spotlight: Efficient and safe single-cell cloning of hPSCs using the CEPT Cocktail
• FAQs: General Guide for Small Molecules

References and Publications

1. NEW! Bertaccini GA, et al. 2025. Visualizing PIEZO1 Localization and Activity in hiPSC-Derived Single Cells and Organoids with HaloTag Technology. [Preprint]. Nature Methods. 2025 Jan 25:2023.12.22.573117
2. Yu Chen, et al. 2021. A Versatile Polypharmacology Platform Promotes Cytoprotection and Viability of Human Pluripotent and Differentiated Cells. Nature Methods. May; 18(5): 528-514
3. Tristan, Carlos A et al. 2021. Robotic high-throughput biomanufacturing and functional differentiation of human pluripotent stem cells. Stem Cell Reports, Volume 16, Issue 12, 3078-3092
4. NIH. 2021. Scientists identify small-molecule cocktail to improve stem cell use in research and disease treatments. Press Release.