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Y-27632 (GMP-grade) increases survival rate of hESCs and iPSCs undergoing cryopreservation. Used to reprogram fibroblasts to mature neurons and used as component of growth media for urothelial organoids. Produced under GMP guidelines and is ideal for cell therapy research.
Y-27632 is a common research tool used in the field of stem cell research for maintenance, differentiation, and the survival of cells following cryopreservation. Y-27632 is used as 3D growth matrix component and included in organoid differentiation media. This small molecule is produced under GMP guidelines and is ideal for cell therapy research.
Y-27632 dihydrochloride is a highly specific inhibitor of Rho-associated coiled-coil protein kinase (ROCK) activity as a cell-permeable, ATP-competitive inhibitor of both ROCK1 (Ki of 220 nM) and ROCK2 (Ki 300 nM) (Ishizaki, et al.). Human embryonic stem cell (ESC) survival is improved through enhanced attachment following single cell dissociation, cryopreservation, and FACS sorting in both feeder-dependent and -independent cultures (Emre, et al.). This enhanced cell attachment, and thus recovery, is boosted through decreased dissociation-induced apoptosis (Watanabe, et al.). Y-27632 dihydrochloride primes human induced pluripotent stem cells (iPSCs) to selectively differentiate towards mesendodermal lineage via epithelial-mesenchymal transition-like modulation.
Description: Y-27632 dihydrochloride, Y-27632, ROCK inhibitor, Y27632, Rho kinase inhibitor Alternative Names: Y-27632 dihydrochloride, Y-27632, ROCK inhibitor, Y27632, Rho kinase inhibitor Applications: Differentiation, cryopreservation, expansion, maintenance, reprogramming, spheroid cultures
As your research program advances into therapeutic applications, making the transition from research-grade to GMP-grade reagents is critical to the success of your project. Captivate Bio can facilitate this transition easier with the availability of both RUO and GMP-grade small molecules that are designed with enhanced quality testing and are suitable for use as ancillary reagents.
Keywords: Y-27632 dihydrochloride, Y27632 ROCK inhibitors, rho-kinase, stem cells, organoids, induced pluripotent stem cells, iPSCs, ROCKI, ROCK1, ROCKII, ROCK2, reprogramming, cryopreservation, differentiation, cell freezing, cell culture media, reprogramming, stem cell survival, gmp small molecules, gmp
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