Y-27632 (2HCl)

Y-27632 is the ROCK star of Stem Cell Research

Y-27632 is a common research tool used in the field of stem cell research for maintenance, differentiation, and the survival of cells following cryopreservation. Y-27632 is used as 3D growth matrix component and included in organoid differentiation media.

Y-27632 dihydrochloride is a highly specific inhibitor of Rho-associated coiled-coil protein kinase (ROCK) activity as a cell-permeable, ATP-competitive inhibitor of both ROCK1 (Ki of 220 nM) and ROCK2 (Ki 300 nM) (Ishizaki, et al.). Human embryonic stem cell (ESC) survival is improved through enhanced attachment following single cell dissociation, cryopreservation, and FACS sorting in both feeder-dependent and -independent cultures (Emre, et al.). This enhanced cell attachment, and thus recovery, is boosted through decreased dissociation-induced apoptosis (Watanabe, et al.). Y-27632 dihydrochloride primes human induced pluripotent stem cells (iPSCs) to selectively differentiate towards mesendodermal lineage via epithelial-mesenchymal transition-like modulation.

Features & Benefits

• High quality guaranteed
• Low price and fast delivery
• Third-party tested and validated
• High purity and consistent activity

Alternative names: Y-27632 dihydrochloride, Y-27632, ROCK inhibitor, Y27632, Rho kinase inhibitor
Applications: Differentiation, cryopreservation, expansion, maintenance, reprogramming, spheroid Culture
Cell types: Neurons, Pluripotent Stem Cells, iPSCs, ESCs

Keywords: Y-27632 dihydrochloride, Y27632 ROCK inhibitors, rho-kinase, stem cells, organoids, induced pluripotent stem cells, iPSCs, ROCKI, ROCK1, ROCKII, ROCK2, reprogramming, cryopreservation, differentiation, cell freezing, cell culture media, reprogramming, stem cell survival

Documents

• Technical Data Sheet
• SDS
• Brochure: Small Molecules for Stem Cell Research
• FAQs: General Guide for Small Molecules

References and Publications

1. NEW! Bertaccini GA, et al. 2025. Visualizing PIEZO1 Localization and Activity in hiPSC-Derived Single Cells and Organoids with HaloTag Technology. [Preprint]. Nature Methods. 2025 Jan 25:2023.12.22.573117
2. Maldonado, M., et al. 2016. ROCK inhibitor primes human induced pluripotent stem cells to selectively differentiate towards mesendodermal lineage via epithelial-mesenchymal transition-like modulation. Stem Cell Res.
3. Xue, ZW, et al. 2012. Rho-associated coiled kinase inhibitor Y-27632 promotes neuronal-like differentiation of adult human adipose tissue-derived stem cells. Chin Med J (Engl).
4. Emre, et al. 2010. The ROCK inhibitor Y-27632 improves recovery of human embryonic stem cells after fluorescence-activated cell sorting with multiple cell surface markers. Plos One.
5. Li, X. et al. 2009. ROCK inhibitor improves survival of cryopreserved serum/feeder-free single human embryonic stem cells. Hum. Reprod.
6. Watanabe, et al. 2007. A ROCK inhibitor permits survival of dissociated human embryonic stem cells. Nature Biotechnology.
7. Tada, S, et al. 2001. A selective ROCK inhibitor, Y27632, prevents dimethylnitrosamine-induced hepatic fibrosis in rats. J Hepatol.
8. Ishizaki, et al. 2000. Pharmacological properties of Y-27632, a specific inhibitor of rho-associated kinases. Molecular Pharmacology.
9. Narumiya, et al. 2000. Use and properties of ROCK-specific inhibitor Y-27632. Methods Enzymol.