MCDB 131, no L-glutamine, no Phenol Red

MCDB 131 Medium contains many components not found in traditional basal media, such as trace elements, putrescine, adenine, thymidine, and higher levels of some amino acids and vitamins. These additions allow the medium to be supplemented with very low levels of serum or defined components. MCDB 131 Medium contains no proteins or growth factors, and is often supplemented with EGF, glutamine, and low levels of Fetal Bovine Serum (FBS) or other cytokines and growth factors. MCDB 131 Medium uses a sodium bicarbonate buffer system, and therefore requires a 5–10% CO2 environment to maintain physiological pH.

This MCDB 131 Medium is manufactured as follows:

With: Sodium Pyruvate

Without: L-glutamine, HEPES, Phenol Red

Cell Types: Human Microvascular Endothelial Cells (HMVEC), Human Omental Microvascular Cells, Hepatocytes, Myocytes, Smooth Muscle Cells