SB203580

SB203580 is a selective ATP-competitive p38 MAPK inhibitor with IC50s of 50 nM and 500 nM for SAPK2a/p38 and SAPK2b/p38β2, respectively. SB203580 inhibits LCK, GSK3β and PKBα with IC50s of 100-500-fold higher than that for SAPK2a/p38. SB203580 does not disrupt JNK activity and is an autophagy and mitophagy activator. SB203580 has been shown to enhance clonal growth of skin epithelial progenitor cells and to stimulate neural stem cell (NSC) proliferation. Additionally, SB203580 has also demonstrated significant inhibition of the proliferation of HNSCC cells both in vitro and in vivo.

Features

• Enhances the growth of mouse embryonic stem (ES) cells (Qi et al.)
• Promotes long-term maintenance of human naïve pluripotent stem cells (Gafni et al.)
• Promotes proliferation of human endothelial progenitor cells (Seeger et al.)
• Enhances differentiation of cardiomyocytes from human ES cells (Gaur et al., Graichen et al.)

Documents

• SB203580 Technical Data Sheet
• Brochure: Small Molecules for Stem Cell Research
• FAQs: General Guide for Small Molecules

References and Publications

• Borsch-Haubold, A.G., et al. (1998) Direct inhibition of cyclooxygenase-1 and -2 by the kinase inhibitors SB 203580 and PD 98059. J Biol Chem 273: 28766-28772. PMID: 9786874.
• Davies SP, et al. Specificity and mechanism of action of some commonly used protein kinase inhibitors. Biochem J. 2000 Oct 1;351(Pt 1):95-105.
• Lali FV, et al. The pyridinyl imidazole inhibitor SB203580 blocks phosphoinositide-dependent protein kinase activity, protein kinase B phosphorylation, and retinoblastoma hyperphosphorylation in interleukin-2-stimulated T cells independently of p38 mitogen- activated protein kinase. J Biol Chem. 2000 Mar 10;275(10):7395- 402.
• Leelahavanichkul K, et al. A role for p38 MAPK in head and neck cancer cell growth and tumor induced angiogenesis and lymph angiogenesis. Mol Oncol. 2014 Feb;8(1):105-18.
• Sato, K., et al. (2008) Inhibitors of p38 mitogen-activated protein kinase enhance proliferation of mouse neural stem cells. J Neurosci Res 86: 2179-2189.
• Gafni O et al. (2013) Derivation of novel human ground state naive pluripotent stem cells. Nature.