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Wnt-3a, Human, HEK293

Wnt-3a Protein, Human is the recombinant human-derived Wnt3a Surrogate, expressed by HEK293, with C-hFc labeled tag. Wnt3a is considered as the essential protein for organoid growth. This Wnt3a is a water-soluble and Fzd-specific alternative Wnt agonist and an alternative to Wnt3a.

Grouped product items
SKU	SIZE	PRICE	QTY
CYK18L	50 ug	$675.00	- +
CYK18F	100 ug	$905.00	- +

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SKU	CYK18
Form	Lyophilized powder
Alternative Names	WNT3A; Wnt Family Member 3A, Wingless-Type MMTV Integration Site Family, Member 3A, Protein Wnt-3a
Species	Human
Source/Origin	HEK293
Tag	C-human IgG1 Fc
Molecular Weight	Approximately 60-75 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
Purity	≥ 95%, as determined by reducing SDS-PAGE.
Formulation	Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.
Endotoxin	<1 EU/μg, determined by LAL method.
Reconstitution	It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Storage	Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Shipping	AMB

Wnt-3a Protein (Wnt3a) is a Wnt recombinant protein that plays an important role in embryonic development, adult tissue homeostasis, and wound repair. This Wnt3a consists of the domain and Fc portion of Wnt3a, helps organoids expand and maintain a source of Wnt. Organoids are a rapidly evolving model system for tissue development, and Wnt3a is considered as the essential protein for organoid growth.

Wnt3a and CHIR99021 are commonly used as Wnt sources in experiments. However, the application of Wnt3a is hampered by Wnt lipidation, and cross-reactivity with Wnt frizzled (Fzd). Therefore, Wnt3a Surrogate protein was used instead of Wnt3a, as a water-soluble and Fzd-specific alternative Wnt agonist. And the Wnt3a Surrogate protein exhibits tissue selectivity consistent with Fzd isoform specificity. Wnt3a Surrogate Protein, Human (HEK293, C-hFc) is the recombinant human-derived Wnt3a Surrogate, expressed by HEK293, with C-hFc labeled tag.

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