RESiVATE™ MSC Expansion Media

Bringing a new generation of cell culture media to the forefront

Introducing RESiVATE™ – a new generation of xeno-free cell culture media designed for activating and expanding high quality human MSCs while giving you ultimate control of your cultures. RESiVATE™ combines premium quality ingredients with trusted manufacturing, setting a new standard for cell culture media in scientific research.

Captivate Bio’s RESiVATE™ MSC Expansion Kit is a cost-effective and growth factor rich culture system that enables scientists the ability to customize culture conditions on the fly. Available with or without the supplement, the RESiVATE™ MSC Basal Medium is a baseline formulation that does not contain growth factors, cytokines, or unknown supplements, leaving you in full control of your experimental conditions.

Features

• Complete medium contains growth factors and supplements to support hMSC growth
• Basal medium provides flexibility with various supplementation strategies (HPL, FBS, or HSA) and cell types including mouse, rat, and human MSCs
• Flexible formats, includes basal medium and supplement
• No need for attachment matrix to plate cells
• Manufactured in the US under cGMP conditions and in ISO-certified facilities

This kit is shipped in two parts:
- RESiVATE™ MSC Basal Medium, 500 mL, store at 2°C to 8°C
- RESiVATE™ HPL Supplement, 27 mL, store at -20°C

The Basal Medium ships at room temperature, while the Supplement ships on dry ice.

Expansion of human bone marrow derived mesenchymal stem cells (BM-MSCs) in RESiVATE MSC Expansion Media is comparable to FBS-containing media in terms of morphology and growth characteristics. Results shown provided by EverCell Bio, a leading stem cell services organization located in Natick, MA, USA.

		BM-MSCs exhibit typical fibroblast-like cell morphology when cultured in RESiVATE MSC Expansion Medium. Results: Expansion of BM-MSCs cultured in RESiVATE MSC Expansion Medium. MSCs were seeded at 7,000 cells/cm2 on day 0 with image captured on day 1 and day 5 during initial passage. Typical “shoalike” morphology of MSCs was observed.
hMSCs cultured in RESiVATE MSC Expansion Media exhibit higher proliferation rates and long-term growth compared to MSCs cultured in FBS-containing media. Results: Isolated BM-MSCs were cultured in RESiVATE MSC Expansion Medium or FBS-containing media on fibronectin for 5 passages. Cell counts and viability was obtained using live/dead stain AO/PI before replating at density of 8,000 cells/cm2.	hMSCs expanded in RESiVATE MSC Expansion Media maintained high protein expression of CD73, CD90 and CD105 after multiple passages. Results: BM-MSCs were cultured in RESiVATE MSC Expansion Medium or control media (10% FBS) for 5 passages before performing phenotypic staining for MSC markers CD73, CD90 and CD105. Protein expression for typical MSC markers was maintained as high or higher compared to MSCs grown in FBS-containing media.	hMSCs cultured in RESiVATE MSC Expansion Media yielded more colonies than MSCs cultured in control media (10% FBS). Results: After 3 passages, BM-MSCs were seeded at 50 cells/cm2 and cultured in RESiVATE MSC Expansion Medium or FBS-containing media for 10 days before fixing and assessing colony formation. More colonies were observed in MSCs cultured in RESiVATE MSC Expansion Media.

Documents

• Product Sheet
• RESiVATE™ Brand Ambassador Program Flyer
• RESiVATE™ Guidelines for Use