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IWP 4 is a potent inhibitor of Wnt/β-catenin signaling (IC50 = 25 nM). Has minimal effect on Notch and Hedgehog signaling pathways. Induces differentiation of cardiomyocytes from human ESCs and iPSCs.
IWP-4 is an inhibitor of the Wnt pathway, specifically targeting membrane-bound O-acyltransferase porcupine (PORCN) with an IC50 of 25 nM. IWP-4 has been an effective component within differentiation protocols of pluripotent stem cells (PSCs) into functional cardiomyocytes when combined with CHIR99021, IWP-2, Activin A, bFGF, and BMP4 (Lian, et al.), as well as in suspension cultures for cardiac differentiation from human PSCs (Chen, et al.). IWP-4 precisely induces expression of myosin light chain in both atrial and ventricular forms for the creation of primitive cardiomyocytes from human embryonic stem cells (ESCs) (Hudson, et al.).
IWP-4 Technical Data Sheet
IWP-4 SDS
Brochure: Small Molecules for Stem Cell Research
FAQs: General Guide for Small Molecules
Noor, et al. 2019. 3D Printing of Personalized Thick and Perfusable Cardiac Patches and Hearts. Adv Sci (Weinh) 6 1900344.
Hoang, et al. 2018. Generation of spatial-patterned early-developing cardiac organoids using human pluripotent stem cells. Nat Protoc. 13 723.
Chen, et al. 2015. Development of a scalable suspension culture for cardiac differentiation from human pluripotent stem cells. Stem Cell Research. 15(2): 365-375.
Narytnyk, et al. 2014. Differentiation of human epidermal neural crest stem cells (hEPI-NCSC) into virtually homogenous populations of DArgic neurons. Stem Cell Rev. 10 316.
Lian, et al. 2013. Directed cardiomyocyte differentiation from human pluripotent stem cells by modulating Wnt/β-catenin signaling under fully defined conditions. Nature Protocols 8(1): 162-175.
Hudson, et al. 2011. Primitive cardiac cells from human embryonic stem cells. Stem Cells and Development. 21(9): 1513-23.
Chen, et al. 2009. Small molecule-mediated disruption of Wnt-dependent signaling in tissue regeneration and cancer. Nat.Chem.Biol. 5 100.
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