IMDM, HEPES

IMDM (Iscove's Modified Dulbecco's Medium) is utilized not only for more specialized cell types but also as the basis for some of the more unique serum-free media formulations. The original formulation designed by Iscove and associates, demonstrated that erythrocytes and macrophage precursor cells (e.g. primary Haemopoietic Cells) could be cultured in a totally defined serum-free medium when supplemented with albumin, lecithin, selenium and transferrin. IMDM is a modification of DMEM (Dulbecco's Modified Eagle's Medium) containing selenium, additional AA's, vitamins and other components. IMDM is a highly enriched synthetic media well-suited for rapidly proliferating and high-density cell cultures in a 5% CO2 atmosphere.

IMDM medium reportedly supports Murine B Lymphocytes, Haemopoietic tissues from Bone Marrow, B-cells stimulated with Lipopolysaccharide, and also T-Lymphocytes and a wide variety of Hybrid cells. When utilized in conjunction with serum, it supports a broad spectrum of Mammalian cell types including but not exclusive to bone marrow, hybridomas and lymphocytes.

This product is manufactured by Sartorius and available in case packs only.

This IMDM is modified as follows:

With

• L-Glutamine
• Phenol Red
• Sodium Bicarbonate
• HEPES

Without:

• Beta Mercaptoethanol
• Alpha-Thioglycerol