HBSS, no Calcium and Magnesium

Maintaining balance in your cell cultures

Hanks’ Balanced Salt Solution (HBSS) is a buffer comprised of inorganic salts and supplemented with glucose as an energy source. HBSS is commonly used in a variety of cell applications such as washing, diluting, and transporting cell or tissue samples. HBSS provides cells with a source of water, essential inorganic ions, and a carbohydrate as an energy source.

Formulations with calcium and magnesium support cell adhesion and are generally used as transport media, or for reagent preparation. Formulations without calcium and magnesium are required for rinsing chelators from the culture before cell dissociation.

This HBSS is modified as follows:

With:
- Phenol Red

Without:
- Calcium
- Magnesium

Custom formats, bulk discounts, and storage programs are also available. All our salt solutions are manufactured under cGMP guidelines and in ISO-certified facilities.

Documents

• Product Information Sheet

References and Publications

1. Relation of Oxygen and Temperature in the Preservation of Tissues by Refrigeration.
   Hanks, J.H. and Wallace, R.E. 1949. Proc. Soc. Exp. Biol. Med., 71:196. PMID: 18134009.