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EGF Protein, Human, E. coli

EGF Protein, Human is the recombinant human-derived EGF protein, expressed by E. coli, with tag free. EGF drives proliferation of the epithelial stem cells.

Grouped product items
SKUSIZEPRICE QTY
GTF05L50 ug
$140.00
- +
GTF05F100 ug
$180.00
- +
GTF05G500 ug
$425.00
- +
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Description
Specifications
More Information
SKUGTF05
FormLyophilized powder
Alternative NamesEGF, Alternative Protein EGF, Epidermal Growth Factor, Beta-Urogastrone, Pro-Epidermal Growth Factor, HOMG4, Epidermal Growth Factor (Beta-Urogastrone); URG
SpeciesHuman
Source/OriginE. coli
TagTag Free
AccessionP01133-1 (N971-R1023)
Gene ID1950 [NCBI]
Molecular WeightApproximately 6-11 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
Purity≥ 95%, as determined by reducing SDS-PAGE.
FormulationLyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.
Endotoxin<1 EU/μg, determined by LAL method.
ReconstitutionIt is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
StorageStored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

EGF proteins act as potent stimulators of growth in a variety of epidermal and epithelial tissues in both in vivo and in vitro settings, while promoting the proliferation of specific fibroblasts in cell culture. This multifaceted protein also acts as a magnesium stimulating hormone, driving magnesium reabsorption in the renal distal tubule through engagement of EGFR and activation of the magnesium channel TRPM6. EGF Protein, Human is the recombinant human-derived EGF protein, expressed by E. coli, with tag free.

EGF drives proliferation of the epithelial stem/progenitor cells through EGFR signaling and is present in essentially every epithelial organoid medium published.

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