DPBS, no calcium, no magnesium, endotoxin-free

Endotoxin-Free DPBS (no calcium, no magnesium) is sterile filtered through a 0.1 micron filter and is considered endotoxin-free (<0.005 EU/ml). DPBS has been used as a wash buffer during the isolation of colonic lamina propria cells and teratoma formation by induced pluripotent stem cells (iPSCs).

General balanced salt solutions can provide an environment that maintains the structural and physiological integrity of cells in vitro. Dulbecco’s Phosphate Buffered Saline (DPBS) is a balanced salt solution used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue samples, diluting cells for counting, or as an inorganic base for standard media preparation.

This DPBS is modified as follows:

• Calcium
• Magnesium
• Phenol Red

Standard packaging includes 1000 mL bottles. Other volumes and bioprocessing bags are available upon request. Endotoxin-Free DPBS is manufactured in cGMP, ISO-certified facilities. Tests include osmolality, pH, stability, and the absence of bacterial/fungal contamination and endotoxin.

To inquire about bulk or custom options, please inquire here.

Documents

• Product Information Sheet
• SDS

References and Publications

1. Plaque formation and isolation of pure lines with Poliomyelitis viruses.
   Dulbecco, R. and Vogt, M. 1954. J. Exp. Med., 98:167.