DAPT (GMP)

Captivate Bio offers a portfolio of GMP-grade small molecules. GMP-grade small molecules are produced under well establish, controlled cGMP guidelines and are used as ancillary reagents in the generation of stem cells and in reprogramming, self-and differentiation workflows for cell therapy applications.

DAPT (GMP-grade) is a γ-secretase inhibitor (with IC50s of 115 and 200 nM for total Aβ and Aβ42, respectively) that can reduce Aβ40 and Aβ42 levels in human primary neuronal cells and brain extracts, DAPT also inhibits γ-secretase complex and indirectly inhibits Notch, a γ-secretase substrate (Dovey, et al). Its activity causes neural cells to commit to neuronal differentiation (Crawford et al) and has been shown to reduce colony-forming efficiency in mouse neural stem cells (Androutsellis-Theotokis et al). DAPT has also been shown to promote differentiation of pancreatic cells from human pluripotent stem cells (D’Amour et al).

Alternative names: GSI-IX; GSI IX, γ-Secretase Inhibitor IX, LY-374973
Applications: induction of neurons from hiPSCs, generation of midbrain dopaminergic neurons from hPSCs
Cell types: Neurons, iPSCs, hepatocytes, retinal organoids

Accelerating Cell Therapy Research Programs

As your research program advances into therapeutic applications, making the transition from research-grade to GMP-grade reagents is critical to the success of your project. Captivate Bio can facilitate this transition easier with the availability of both RUO and GMP-grade small molecules that are designed with enhanced quality testing and are suitable for use as ancillary reagents.

GMP Features

• Enhanced quality testing and documentation
• High purity and consistent activity
• Reduce risk of unwanted genetic material
• Easily scalable, available in large volumes
• Manufactured following ICH Q7 guidelines

Quality Assurance

• QC tests include: purity, water content, residual solvents, bioburden, endotoxin, elemental impurities, and solubility
• Documentation includes: Certificate of Analysis (CoA), Safety Data Sheet (SDS), Certificate of Origin (CoO), TSE/BSE Statement, Technical Data Sheet, and Declaration of GMP Compliance

Keywords: DAPT (GMP), γ-secretase, Gamma secretase, GMP, cell therapy, hiPSC, myoblast, neurons, Inhibitor, stem cell differentiation