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IWP 4 is a potent inhibitor of Wnt/β-catenin signaling (IC50 = 25 nM). Has minimal effect on Notch and Hedgehog signaling pathways. Induces differentiation of cardiomyocytes from human ESCs and iPSCs.

SB203580 is a selective ATP-competitive p38 MAPK inhibitor. Enhances the growth of mouse ES cells and enhances differentiation of cardiomyocytes from human ES cells.