15 mL PBMC Separation Tubes

Captivate Bio offers a new family of NEST Peripheral Blood Lymphocyte Separation Tubes. These tubes are specialized tube used to isolate peripheral blood mononuclear cells (PBMCs), including lymphocytes and monocytes, from whole blood or bone marrow. These tubes simplify the isolation process allowing for the rapid collection of high-purity, viable PBMCs through a simple centrifugation and decanting step.

Features:

- Available in both 15 mL and 50 mL sizes.
- Rapid separation and purification for PBMCs within 15 min.
- Simple to use: simply decant the centrifuge tube.
- E-beam sterilized with SAL=106.
- Pyrogen free, nucleases free.

Product Selection Chart:

Captivate Bio's NEST PBMC Separation Tubes are available in bulk with further discounting. Contact our customer service team for a free quote.

Application Protocol

Instructions:

• Bring your separation solution to room temperature (away from light).
• Add separation solution to tube: for a 15 mL tube, add approximately 4 mL separation solution and for a 50 mL tube, add approximately 13 mL separation solution. Please ensure the separation solution is on top of the nested container throughout the entire procedure.
• Add the drawn sample into the room temperature separation tube. Tip: adding saline to dilute the specimen is not required but can help improve the separation result.
• Slowly pour or pipette the anticoagulated sample (blood or bone marrow, diluted with saline if needed) along the tube wall slowly: for a 15 mL separation tube, 4-9 mL of the sample is recommended; for a 50 mL separation tube, 13-30 mL of the sample is recommended.
• Centrifuge at room temperature with a centrifugal force of 1200 x g for 10 minutes, then turn off the centrifuge. For samples left for more than 24h, longer centrifugation time is recommended.
• Liquid separation condition after centrifugation (from top to bottom):
  A. plasma
  B. enriched cell fractions (intermediate phase contains lymphocytes/PBMCs cells)
  C. separation solution
  D. nested container
  E. precipitation (erythrocytes and granulocytes).
  Collect or directly discard the plasma layer of 5-10 mm above the enriched cells to prevent the enriched cells from re-contamination by platelets.
• Collect the enriched cells (lymphocytes/PBMCs cells) and pour the supernatant from the separation tube into another clean centrifuge tube, during which the nested container works to prevent the enriched cells from re-contamination by erythrocytes and granulocytes. Don’t invert the separation tube for more than 2s.
• Wash the enriched cells (lymphocytes/PBMCs cells) with PBS then centrifuge them at 250 xg for 10 min.
• Repeat the washing and re-suspend cells with 5 mL PBS.