Introducing RESiVATE™ – a new generation of xeno-free cellculture media designed for the rapid expansion of high quality human MSCs while giving you ultimate control of your cultures.
Captivate Bio’s RESiVATE™ MSC Expansion Kit is a cost-effective and growth factor rich culture system that enables scientists the ability to customize culture conditions on the fly. Available with or without the supplement, the RESiVATE™ MSC Basal Medium is a baseline formulation that does not contain proteins or unknown supplements, leaving you in full control of your experimental conditions.
BM-MSCs exhibit typical fibroblast-like cell morphology when cultured in RESiVATE MSC Expansion Medium.Results: Expansion of BM-MSCs cultured in RESiVATE MSC Expansion Medium. MSCs were seeded at 8,000 cells/cm2 on day 0 with image captured on day 1 and day 5 during initial passage. Typical “shoalike” morphology of MSCs was observed.
Expansion of human bone marrow derived mesenchymal stem cells (BM-MSCs) in RESiVATE MSC Expansion Media is comparable to FBS-containing media in terms of morphology and growth characteristics. Results shown provided by EverCell Bio, a leading stem cell services organization located in Natick, MA, USA.
hMSCs cultured in RESiVATE MSC Expansion Media exhibit higher proliferation rates and long-term growth compared to MSCs cultured in FBS-containing media.
Results: Isolated BM-MSCs were cultured in RESiVATE MSC Expansion Medium or FBS-containing media on fibronectin for 5 passages. Cell counts and viability was obtained using live/dead stain AO/PI before replating at density of 8,000 cells/cm2.
hMSCs expanded in RESiVATE MSC Expansion Media maintained high protein expression of CD73, CD90 and CD105 after multiple passages.
Results: BM-MSCs were cultured in RESiVATE MSC Expansion Medium or control media (10% FBS) for 5 passages before performing phenotypic staining for MSC markers CD73, CD90 and CD105. Protein expression for typical MSC markers was maintained as high or higher compared to MSCs grown in FBS-containing media.
hMSCs cultured in RESiVATE MSC Expansion Media yielded more colonies than MSCs cultured in control media (10% FBS).
Results: After 3 passages, BM-MSCs were seeded at 50 cells/cm2 and cultured in RESiVATE MSC Expansion Medium or FBS-containing media for 10 days before fixing and assessing colony formation. More colonies were observed in MSCs cultured in RESiVATE MSC Expansion Media.
This Research Spotlight takes a look at a study in Scientific Reports where researchers compare six commercially available media in the growth and expansion of BM-MSCs. Two media are found to rise to the top.
This article reviews some of the key terminology in regenerative medicine used to define various areas of cell culture media manufacturing.
Try Captivate Bio’s new RESiVATE™ MSC Expansion Media – a new generation of xeno-free cell culture media. Get a free sample and start testing today.
