Thiazovivin (GMP)

Captivate Bio offers a portfolio of GMP-grade small molecules. GMP-grade small molecules are produced under well establish, controlled cGMP guidelines and are used as ancillary reagents in the generation of stem cells and in reprogramming, self-and differentiation workflows for cell therapy applications.

Thiazovivin (GMP) is produced under GMP guidelines and is a potent and selective inhibitor of Rho kinase (ROCK) activity, a key player in cytoskeletal contraction and rearrangement. As ROCK inhibition occurs, human embryonic stem cell survival increases through enhanced cell-ECM attachment via E-cadherin signaling (Xu, et al.), in addition to allowing for an environment for improved human iPSCs from fibroblasts when used in combination with ALK5 inhibitor SB 431542 and MEK inhibitor PD0325901 (Lin, et al.). The addition of Thiazovivin also creates more effective conditions for the reprogramming of cord blood mononuclear cells into iPSCs (Hu, et al.).

Applications: cellular reprogramming, mouse embryonic fibroblasts into chemically induced neural stem cells, improves the efficiency of iPSC generation
Molecular name: Thiazovivin, ROCK inhibitor
Purity: Greater than 95% by LCMS

Accelerating Cell Therapy Research Programs

As your research program advances into therapeutic applications, making the transition from research-grade to GMP-grade reagents is critical to the success of your project. Captivate Bio can facilitate this transition easier with the availability of both RUO and GMP-grade small molecules that are designed with enhanced quality testing and are suitable for use as ancillary reagents.

GMP Features

• Enhanced quality testing and documentation
• High purity and consistent activity
• Reduce risk of unwanted genetic material
• Easily scalable, available in large volumes
• Manufactured following ICH Q7 guidelines

Quality Assurance

• QC tests include: purity, water content, residual solvents, bioburden, endotoxin, elemental impurities, and solubility
• Documentation includes: Certificate of Analysis (CoA), Safety Data Sheet (SDS), Certificate of Origin (CoO), TSE/BSE Statement, Technical Data Sheet, and Declaration of GMP Compliance

Keywords: Thiazovivin, Thiazovivin supplier, stem cells, reprogramming, dedifferentiation, induced pluripotent stem cells, ipscs, hesc, neural, fibroblasts, reprogramming, rho-kinases, cell proliferation

Documents

• Technical Data Sheet
• Flyer: GMP Small Molecules for Cell Therapy Research
• SDS
• Brochure: Small Molecules for Stem Cell Research
• FAQs: General Guide for Small Molecules

References and Publications

1. Hu, et al. 2011. Efficient generation of transgene-free induced pluripotent stem cells from normal and neoplastic bone marrow and cord blood mononuclear cells. Blood. 117(14): 109-119.
2. Lin, et al. 2009. A chemical platform for improved induction of human iPSCs. Nature Methods. 6: 805-808.
3. Xu, et al. 2010. Revealing a core signaling regulatory mechanism for pluripotent stem cell survival and self-renewal by small molecules.