TGF-beta 3, Human, HEK293

TGF-beta 3 (transforming growth factor beta 3, TGF-β 3) is a member of the TGF-β superfamily, and is an important growth factor in embryonic development and adult wound healing and tissue homeostasis. TGF-beta 3 plays a key role in regulating cell migration, apoptosis, epithelial-mesenchymal transition (EMT), angiogenesis, extracellular matrix (ECM) production and remodeling, and modulation of immune function. TGF-beta 3 is highly conserved across species, with mouse, rat, and human TGF-beta 3 showing >97% sequence homology in sequence.

Recombinant TGF-beta 3 protein is involved in cell differentiation, development, tissue regeneration, and tissue repair. TGF-beta 3 has been used as a potent replacement for TGF-beta 1 in culture media to maintain undifferentiated human pluripotent stem cells (hPSC), shown to enhance glycosaminoglycan production of mesenchymal stem/stromal cells (MSCs), and stimulate the expression of fibroblast growth factors 2 and 18.

TGF-beta 3 binds to the type II receptor (TβRII) and activates signal transduction through SMAD2/3 proteins.

Cross-reactivity: Mouse, rat

Applications: Pluripotent stem cell (hPSC) culture, pluripotency maintenance, differentiation, mesenchymal stem cell (MSC) culture, immunology

Cell types: hPSCs, MSCs, T cells, B cells

Features & Benefits

• Recombinant human protein expressed in human cells (HEK293)
• Native protein post-translational modifications, including protein folding, maturation and glycosylation
• Improved activity and stability gained from native protein structure

Documents

• Recombinant Proteins Product Information Sheet

References & Publications

1. Massagque, J. How cells read TGF-beta signals. Nat Rev Mol Cell Bio. 2000; 1(3):169-78.
2. Laverty et al. TGF-beta3 and cancer: a review. Cytokine Growth Factor Rev. 2009; 20(4):305-17.
3. Toshihiko et al. Reevaluation of Pluripotent Cytokine TGF-β3 in Immunity. Int J Mol Sci. 2018; 19(8):2261.
4. Letterio and Roberts. Regulation of immune responses by TGF-beta. Annu Rev Immunol. 1998; 16:137-61.
5. James et al. Differential effects of TGF-beta 1 and TGF-beta 3 on chondrogenesis in posterofrontal cranial suture-derived mesenchymal cells in vitro. Plast Reconstr Surg. 2009; 123(1):31-43.