Medium 199, with L-glutamine

Medium 199 was originally developed for nutritional studies of chick embryo fibroblasts and has broad species applicability, particularly for cultivation of non-transformed cells. Medium 199 is widely used in virology, vaccine production, and in vitro cultivation of primary explants of mouse pancreatic epithelium, and rat lens tissues.

This Medium 199 is modified as follows:

with:
- Phenol Red
- Earle's Salts

without:
- L-glutamine
- HEPES

Compared to other basal media, Medium 199 contains unique components, including adenine, adenosine, hypoxanthine, thymine, and additional vitamins. Medium 199 with Earle's salts is designed for use in a CO2 incubator and Medium 199 with Hanks' for use without CO2.