CRYOVATE™ SF Freezing Medium

Freeze today. Accelerate tomorrow.

Captivate Bio’s CRYOVATE™ SF Freezing Medium is a 2X, serum-free cryopreservation medium that allows for the optimal storage of human and animal cells. This formulation contains DMSO and other cryoprotectants to ensure cell integrity and viability.

Cells cryopreserved using CRYOVATE™ SF show levels of viability and attachment (adherent cells) that are comparable to cells preserved in DMSO and FBS. CRYOVATE™ SF can be used for both cells cultured in serum-supplemented growth medium as well as cells grown under serum-free conditions. This product has applications for cryopreservation, xeno-free cell culture, cell growth, and viability.

Features:

• Defined, animal-component free, and protein free
• Does not contain phenol red or serum
• Preserves viability, attachment, and cell growth
• Prepared with ACS grade (Research Grade) components
• Manufactured in the US in ISO-certified facilities

Directions for use:
To begin, start by cleaning and disinfecting the outside of the CRYOVATE SF Medium bottle before opening.

1. Dilute the 2X formulation and create a 1:1 solution with either a conditioned medium or a fresh serum-free medium before use.
2. Harvest cells according to standard protocols ensuring high viability (>90%).
3. Resuspend the cells in the diluted 1X CRYOVATE SF Medium at the recommended cell density (typcially 1 - 10 x 106 cells/mL, depending on cell type).
4. Mix suspension thoroughly, avoiding bubbles, and aliquot 1 mL into prelabeled, cryogenic vials.
5. Incubate vials at 2 - 8°C for 10 minutes.
6. Freeze cells using appropriate cryopreservation methods. Transfer to a -80°C overnight, then transfer to liquid nitrogen the following day for long-term storage.

Keywords: cryopreservation, cryoprotectant, freezing medium, cell bank preparation, serum-free freezing media, stem cell freezing, biobanking, CART-T freezing, primary cell cryopreservation

Documents

• Product Information Sheet
• Guidelines for Use
• Safety Data Sheet (SDS)