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Mycoplasma are one of the most common, yet elusive, contaminants of mammalian cell cultures. As the smallest known free-living organism, mycoplasma is a pervasive, parasitic species of highly infectious bacteria that are estimated to contaminate between 15-35% of all continuous cell cultures worldwide.
Captivate Bio offers an easy-to-use EZ-PCR™ Mycoplasma Detection Kit for highly sensitive cells as well as CAPTiCLEAN™ prevention and disinfectant solution for your CO2 incubator water trays. The EZ-PCR Kit is used to detect over 90 different species, to monitor cell line health, to monitor contamination in shared lab spaces, and to rule out that mycoplasma is not interfering with your experiments.
Routinely used by labs worldwide, this sample was provided by the WiCell Research Institute. Eight total reactions, including six samples, one negative control, and one positive control were tested, each of which contain the internal control to rule out PCR inhibition (i.e. false negatives). As shown, Sample 4 produced a mycoplasma-positive band at 270bp in addition to the internal control band at 357bp, while Samples 1, 2, 3, 5, and 6 produced only internal control bands and are thus negative for mycoplasma. Image courtesy of WiCell Research Institute.
Protect your cells by downloading our comprehensive new eBook. There have been thousands of articles, guides, and resources published about mycoplasma contamination. Here, we pulled together many of the main themes to help you fight cell culture contamination in your own lab.
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Captivate Bio's CAPTiCLEAN™ is a highly effective disinfection solution for the treatment and prevention of contamination in CO2 incubator water trays. Add 10 mL per liter. Save when you buy case packs. Each bottle will treat water trays for up to 4-6 weeks.
Highly-sensitive PCR assay designed for the simple and fast detection of mycoplasma contamination in cell cultures. Includes ready-to-use reagents to perform 20 tests, each with internal control.
September 2025. Check out the newest top 10 tips on keeping your lab clean from Captivate Bio. Get tips on water quality and cell line testing. Read now.
This article provides an introduction to mycoplasma, methods for detecting mycoplasma contamination in cell cultures, and the importance of testing cell lines.
Learn about the most common mycoplasma, review various testing methods, and explore tips and tricks for keeping your lab safe.
