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This Research Spotlight takes a look at a study in Scientific Reports where researchers compare six commercially available media in the growth and expansion of BM-MSCs. Two media are found to rise to the top.
When used to enhance hPSC survival and clonal expansion during passaging, Trans-ISRIB is used at a final concentration of 0.7 µM in cell culture media.
Trans-ISRIB, also known as ISRIB trans-isomer, is an integrated stress response (ISR) inhibitor that acts downstream of PERK to target interactions between eIF2α and eIF2B, potently reversing the effects of eIF2α phosphorylation.
Chroman 1 is a highly potent and selective small molecule ROCK inhibitor (both ROCK 1 and ROCK 2), with greater effectiveness at lower doses than Y27632. Chroman 1 has been shown to improve survival of human pluripotent stem cells and enhance recovery of cells after cryopreservation.
When used to enhance hPSC survival and clonal expansion during passaging, Emricasan is used at a final concentration of 5 µM in cell culture media.
Yes. Emricasan (dosed at 5 µM for 24 hours at each passage in combination with Chroman 1) was shown to be safe for use in routine passaging of hPSCs dissociated as single cells.
