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The CET Cocktail is composed of the small molecule components of the published CEPT cocktail (Chen et al. Nat Methods 2021). These 3 small molecules, in combination with polyamine supplement, were shown in the study to improve cytoprotection of a variety of hPSC lines.
While the treatment duration may vary across applications, the small molecules in the CET Cocktail are intended to be used according to the CEPT supplementation strategy for improved human pluripotent stem cell survival and viability in culture. (See Chen et al. Nat Methods 2021 for more information on CEPT.
Chroman 1 is a more effective ROCK inhibitor shown to improve cell survival and recovery of hPSCs after cryopreservation. Learn how Chroman 1 compares to Y-27632 in cultures in this short blog post.
This Protocol Spotlight highlights the work of researchers from The Salk Institute who developed a novel strategy for directly differentiating and generating pancreatic beta cells from hPSCs with high efficiency and minimal byproduct cells.
CEPT supplementation improves hPSC single-cell passaging, differentiation, cryopreservation, cloning, and other manipulations. Meet the new CET Cocktail from Captivate Bio.
Over time, ROCK inhibitors have been shown to boost hPSC viability and survival. This guide summarizes the most commonly used small molecule ROCK inhibitors for stem cell cultures.
