RPMI 1640 Medium, no L-glutamine

RPMI Medium 1640 has been specifically developed for the long-term culture of blood cells, the culture of normal and abnormal human leukocytes (e.g. neoplastic WBC's) and is now used as a general medium (with serum) for hybridoma cultures. Roswell Park Memorial Institute (RPMI) 1640 Medium has also been found suitable for a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas.

This RPMI is modified as follows:

With:

• Phenol Red
• Sodium Bicarbonate

Without:

• L-glutamine
• Glucose

Documents

• Product Information Sheet
• Media Formulation

References

1. Moore GE, et al. 1967. Culture of normal human leukocytes. JAMA. 199 (8): 519–524. PMID 4960081.