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A83-01

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A83-01 molecule facilitates stem cell reprogramming and differentiation of hPSCs. A83-01 is supplied as a lyophilized powder.

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SML23B 10 mg $175.00
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Description
Specifications
More Information
SKUSML23B
Size10 mg
Molecular NameA83-01
FormPowder
Alternative NamesActivin/NODAL/TGFβ pathway inhibitor
Chemical Name3-(6-methyl-2-pyridinyl)-N-phenyl-4-(4-quinolinyl)-1H-Pyrazole-1-carbothioamide
Chemical FormulaC25H19N5S
Molecular Weight421.52
CAS Number909910-43-6
PurityGreater than 98% by HPLC
Physical AppearanceOff-white to light yellow (solid)
TargetALK5; ALK4; ALK7
Shelf-Life≥ 2 years (powder)
ShippingRoom Temperature
StorageStore at -20°C
Quality StatementThis product is for Research Use Only and is not intended for therapeutic or diagnostic use.

A83-01 is a selective inhibitor of activin receptor-like kinase (ALK), including the transforming growth factor-β (TGFβ) type I receptor ALK5, the Activin/Nodal receptor ALK4, and the type 1 nodal receptor ALK71. This molecule is more potent than SB431542 in its inhibition of ALK4, 5, and 7, and only weakly inhibits ALK1, 2, 3, and 6. A83-01 inhibits the TGFβ-induced epithelial-to-mesenchymal transition (EMT) via the inhibition of Smad2 phosphorylation2.

A83-01 facilitates the conversion of epiblast stem cells to the naїve pluripotent state2. and in combination with PD0325901, A83-01 enables OCT4-mediated reprogramming3.

Alternative names: Activin/NODAL/TGFβ pathway inhibitor
Applications: Pluripotent Stem Cells; reprogramming, differentiation
Cell types: Pluripotent Stem Cells, iPSCs, ESCs

Features & Benefits
  • More potent ALK 5 inhibitor than SB431542
  • Enables OCT4-mediated reprogramming in combination with PD02158901
  • Inhibits specification to mesoderm and endoderm
Solubility Table

Stock Concentration Compound Mass
1 mg 5 mg 10 mg
1 mM 2.3724 mL 11.8618 mL 23.7237 mL
5 mM 0.4745 mL 2.3724 mL 4.7447 mL
10 mM 0.2372 mL 1.1862 mL 2.3724 mL
50 mM 0.0475 mL 0.2372 mL 0.4745 mL
 Solvent Volume

Preparation & Reconstitution
  1. From the lyophilized compound, prepare a concentrated stock solution using the appropriate solvent (DMSO), according to the solubility table or custom calculations.

    Example: To generate a 5 mM concentrated stock solution of A85-01, add 2.37 mL of DMSO to 5 mg of A83-01.
  2. Ensure the compound is completely dissolved in the solvent. This may require gentle warming and/or vortexing/sonication to fully reconstitute the compound.
  3. Aliquot the concentrated stock solution in single-use volumes, and either use immediately or freeze at -20°C or -80°C for later use. Avoid freeze/thaw cycles.
  4. Concentrated stock solutions are designed to be diluted just prior to use (e.g. 1:1000 dilution in cell culture medium). For use in cell culture, warm the medium just prior to adding the reconstituted compound.

Resources
Documents
References and Publications
  1. Tojo, M., et al. The ALK-5 inhibitor A-83-01 inhibits Smad signaling and epithelial-to-mesenchymal transition by transforming growth factorβ. Cancer Sci 96: 791-800 (2005).
  2. Li, W., et al. Generation of rat and human induced pluripotent stem cells by combining genetic reprogramming and chemical inhibitors. Cell Stem Cell 4: 16-19 (2009).
  3. Zhou et al. Conversion of mouse epiblast stem cells to an earlier pluripotency state by small molecules. J Bio Chem 285(39); 29676-80 (2010).
  4. Zhu et al., Reprogramming of human primary somatic cells by OCT4 and chemical compounds. Cell Stem Cell 7(6): 651-5 (2010).
  5. Zhang et al. Pharmacological reprogramming of fibroblasts into neural stem cells by signaling-directed transcriptional activation. Cell Stem Cell 18(5): 653-67 (2016).
  6. Taniguchi Y, et al. Enhanced antitumor efficacy of folate-linked liposomal Adriamycin with TGF-β type I receptor inhibitor. Cancer Sci. 2010 Oct;101(10):2207-13.
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