My Cart

Y-27632 (2HCl)

Rating:
100% of 100

Y-27632 is a cell-permeable, highly potent and selective inhibitor of Rho-associated, coiled-coil containing protein kinase (ROCK). Increases survival rate of human embryonic stem (hES) cells and iPSC undergoing cryopreservation. Used to reprogram fibroblasts to mature neurons and used as component of growth media for urothelial organoids.

  • Buy 2 for $62.50 each and save 50%
Simple product items
SKUSIZEPRICEQTY
SML13B - $125.00
- +
Details
Resources
FAQs
Reviews 1
Description
Specifications
More Information
SKUSML13B
Molecular NameY-27632 (2HCl)
Size10 mg
FormPowder
Alternative NamesY-27632 dihydrochloride, Y-27632, ROCK inhibitor, Y27632, Rho kinase inhibitor
Chemical Name4-[(1R)-1-aminoethyl]-N-4-pyridinyl-trans-cyclohexanecarboxamide, dihydrochloride
Chemical FormulaC14H23Cl2N3O
Molecular Weight320.26
CAS Number129830-38-2
PubChem ID9901617
PurityGreater than 95% by HPLC
Physical AppearanceWhite to off-white (Solid)
Shelf-Life≥ 2 years (powder)
ShippingRoom Temperature
StorageStore at -20°C
Quality StatementThis product is for Research Use Only and is not intended for therapeutic or diagnostic use.
Small Molecules for Stem Cell Research

Y-27632 is a common research tool used in the field of stem cell research for maintenance, differentiation, and the survival of cells following cryopreservation. Y-27632 is used as 3D growth matrix component and included in organoid differentiation media.

Y-27632 dihydrochloride is a highly specific inhibitor of Rho-associated coiled-coil protein kinase (ROCK) activity as a cell-permeable, ATP-competitive inhibitor of both ROCK1 (Ki of 220 nM) and ROCK2 (Ki 300 nM) (Ishizaki, et al.). Human embryonic stem cell (ESC) survival is improved through enhanced attachment following single cell dissociation, cryopreservation, and FACS sorting in both feeder-dependent and -independent cultures (Emre, et al.). This enhanced cell attachment, and thus recovery, is boosted through decreased dissociation-induced apoptosis (Watanabe, et al.). Y-27632 dihydrochloride primes human induced pluripotent stem cells (iPSCs) to selectively differentiate towards mesendodermal lineage via epithelial-mesenchymal transition-like modulation.

Alternative names: Y-27632 dihydrochloride, Y-27632, ROCK inhibitor, Y27632, Rho kinase inhibitor
Applications: Differentiation, cryopreservation, expansion, maintenance, reprogramming, spheroid Culture
Cell types: Neurons, Pluripotent Stem Cells, iPSCs, ESCs

Features & Benefits
  • High quality guaranteed
  • Low price and fast delivery
  • Third-party tested and validated
  • High purity and consistent activity
FAQs

What stem cell culture applications can benefit from Y27632?

Y27632 is commonly used to improve cell survival during the routine culture of human pluripotent stem cells (hPSCs), as well as many other sensitive cell types. In addition to routine passaging of hPSCs (both embryonic and induced), common applications include cellular reprogramming/iPSC generation, single cell cloning and gene editing, cryopreservation and recovery, isolation and culture of cancer stem cells, reproductive biology, and transplant medicine.

What is the mechanism of action for Y27632?

Y27632 is a small molecule inhibitor of the Rho-associated coiled-coil containing kinase (ROCK) that improves survival of human pluripotent stem cells. Y27632 improves cell survival by blocking the cell contraction that leads to cell death by apoptosis and anoikis by inhibiting the ROCK pathway.

Reviews 1
Write Your Own Review
You're reviewing:Y-27632 (2HCl)
Your Rating