Small Molecules of Stem Cell Research
SB431542 is a highly-potent and -selective inhibitor of the TGF-β type I receptor, with specific inhibition of ALK5 (IC50 of 94 nM) and its closest relatives ALK4 and ALK7 as part of the TGF-β/Activin/Nodal pathway. SB431542 has been shown to be a highly efficient additive for inducing neural differentiation of embryonic stem cells (ESCs) in a rapid manner when combined with Noggin (Chambers, et al.), or Noggin and LDN193189 (Edri, et al.), promoting the self-renewal of primitive neuroepithelia with hLIF and CHIR99021 (Li, et al. 2011), as well as the derivation of functional neurons from human pluripotent stem cells (PSCs) (Qi, et al.). Additionally, SB431542 allows for the enhanced differentiation of PSCs into cardiomyocytes (Kattman, et al.).
|Alternative Names||SB-431542, SB 431542|
|Chemical Name||Benzamide, 4-[4-(1,3-benzodioxol-5-yl)-5-(2-pyridinyl)-1H-imidazol-2-yl]-|
|Purity||Greater than 95% by LCMS|
|Physical Appearance||Yellow to pink (Solid)|
|Shelf-Life||≥ 2 years (powder)|
|Storage||Store at -20°C|
|Quality Statement||This product is for Research Use Only and is not intended for therapeutic or diagnostic use.|
References and Publications
Qi, et al. 2017. Combined small-molecule inhibition accelerations the derivation of functional cortical neurons from human pluripotent stem cells. Nature Biotechnology 35(2): 154-163.
Edri, et al. 2015. Analysing human neural stem cell ontogeny by consecutive isolation of Notch active neural progenitors. Nature Communications 6(6500).
Kattman, et al. 2011. Stage-specific optimization of activin/nodal and BMP signaling promotes cardiac differentiation of mouse and human pluripotent stem cell lines. Cell Stem Cell. 8(2):228-240.
Li, et al. 2011. Rapid induction and long-term self-renewal of primitive neural precursors from human embryonic stem cells by small molecule inhibitors. PNAS. 108(20): 8299-304.
Chambers, et al. 2009. Highly efficient neural conversion of human ES and iPS cells by dual inhibition of SMAD signaling. Nature Biotechnology 27:275-280.