Small Molecules for Stem Cell Research
Retinoic Acid (all-trans) is a metabolite of vitamin A that acts as a ligand for nuclear retinoic acid receptors (RARs, specifically RARα/β/γ) with an IC50 of 14nM and has a major effect on the differentiation and patterning of stem cells, and therefore in the development process (Rhinn & Dolle). In the process of differentiating mouse embryonic stem cells (ESCs), Retinoic Acid has been shown to promote the growth of glial cells and functional neurons in culture (Fraichard, et al.).
Furthermore, Retinoic Acid has played an important role in protocols involving the differentiation of human pluripotent stem cells (PSCs) into functional pancreatic β cells when combined with CHIR99021, SANT-1, Y27632, Compound E, RepSox, Triiodothyronine Salt, and other growth factors (Pagliuca, et al.).
|Molecular Name||Retinoic Acid|
|Alternative Names||Retinoic acid (AT), Retinoic acid (All-trans), All-trans retinoic acid (ATRA), NSC 122758, Trans retinoic acid, Tretinoin, Vitamin A acid, RA|
|Chemical Name||(all-E)-3,7-Dimethyl-9-(2,6,6-trimethyl-1-cyclohexen-1-yl)-2,4,6,8-nonatetraenoic acid|
|Purity||Greater than 95% by LCMS|
|Physical Appearance||Light yellow to yellow (Solid)|
|Target||RAR/RXR; PPAR; Endogenous Metabolite|
|Shelf-Life||≥ 2 years (powder)|
|Storage||Store at -20°C sealed, to protect from light and moisture|
|Quality Statement||This product is for Research Use Only and is not intended for therapeutic or diagnostic use.|
References and Publications
Pagliuca, et al. 2014. Generation of functional human pancreatic β cells in vitro. Cell 159:428-439.
Rhinn & Dolle. 2012. Retinoic acid signaling during development. Development. 139: 843-858.
Fraichard, et al. 1995. In vitro differentiation of embryonic stem cells into glial cells and functional neurons. J of Cell Science. 108: 3181-3188.