Human pluripotent stem cells (hPSCs) have extensive self-renewal capacity in culture, yet are highly sensitive to their in vitro environment, especially when cells undergo stressful culture manipulations, such as dissociation as single cells during routine passaging or clonal culture. Stressful culture processes such as these often yield poor cell survival. Clinical translation of hPSCs requires well-controlled, safe, and efficient strategies for cell culture expansion and large-scale production, clonal manipulation, cryopreservation, and directed differentiation.
This Research Spotlight reviews an innovative and targeted high-throughput screen and assessment of compounds has found that supplementation with CEPT, the combination of Chroman 1, Emricasan, a Polyamine solution, and Trans-ISRIB, can be safely used in research, regenerative medicine, cryobiology, and drug development applications to improve the viability and expansion of hPSCs in culture, as well as improve functionality of multiple derived cell types.
The Spotlight
Keywords: Pluripotent stem cell culture, long-term culture, single-cell dissociation, clonal cell expansion, iPSC reprogramming
Cell type(s): hPSCs, hiPSCs, hESCs
Experiment purpose: A new targeted high-throughput analysis to identify and characterize a synergistic combination of small molecules and culture additives that can be safely used in research
and regenerative medicine applications to improve the viability and expansion of hPSCs in culture.
Product Highlights
The Captivate Bio CET Cocktail is comprised of the small molecule components in the CEPT cocktail: Chroman 1, Emricasan, and Trans-IRIB (CET).
- Enhances stem cells survival and expansion
- Promotes clonal growth of genetically stable hPSCs
- Provides comprehensive cytoprotection