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A major step in harvesting cells for chromosome karyotyping is treatment with a hypotonic saline solution to increase cell volume. Hypotonic solutions work by creating a concentration gradient across the cytoplasmic membrane and water then rushes in by active transport.
Potassium Chloride (KCl, or potassium salt) is a hypotonic solution prepared in water for use in the preparation of blood lymphocyte chromosomes, the hypotonic treatment allows the cells to take in water and swell. At this step, the cells resemble balloons ready to burst and release their contents, which is the goal of this procedure.
This product is manufactured by Sartorius and available in case packs only.
PDF | Download Captivate Bio's 2024 New Products & Services brochure. Highlighting new reagent sourcing, storage services, salt solutions, water, cytokines and growth factors to support life science research.
PDF | EverCell Bio and Captivate Bio have teamed up to provide a single source solution for stem cell modeling services and ongoing product support to accelerate translational and therapeutic discoveries. Download our brochure here.
This research spotlight highlights the superior cryoprotective capacity of the CEPT method and the survival and functionality of preserved hPSCs.
