PLTGold® Human Platelet Lysate
A growth factor rich, xeno-free, heparin-free supplement that is a superior alternative to FBS. Available in research and clinical grades.
Superior cell performance
Human Platelet Lysate (HPL) provides superior cell performance and cell expansion when compared to other supplements such as FBS or chemically defined media. PLTGold® HPL is a revolutionary xeno-free, heparin-free supplement which provides rapid cell expansion with proven success across various cell types including MSCs and immune cells. Available in research and clinical grades, PLTGold® is an excellent supplement to cell culture media and for use in both 2D and 3D culture systems with a focus on research and cell therapy applications.
- Lot-to-lot consistency allowing for reproducible manufacturing
- Rapid cell expansion when compared to FBS or chemically-defined media
- Sourced from FDA-registered blood centers
- FDA Drug Master File available
Figure 2. Real time imaging of adipose-derived MSC growth using PLTMax® or PLTGold® Human Platelet Lysate as a media supplement showed increased cell growth kinetics compared to cells grown in medium supplemented with FBS or Human AB Serum.
|Intended use||Media supplement, cell culture, cell therapy research, drug discovery, MSCs, T Cells, Hematopoietic Stem Cells, and iPSCs|
|Osmolality||250 - 330 mOsm/kg|
|Endotoxin||< 10.0 EU/mL (available on clinical grade only)|
|Shelf-Life||36 months from date of manufacture|
|pH||6.8 - 7.8|
|Storage||- 20 °C|
|Platelet Testing||Infectious diseases including HIV-1, HIV-2, HIV-NAT, HCV, HBsAg, and serologic tests for Syphilis, ZIKV NAT, WNV NAT|
|Total Protein||4.0 - 6.5 g/dL|
|Source||US donors, AABB-accredited and FDA-registered blood banks|
- Human Platelet Lysate FAQs
- POSTER: Gandhi JK, et al. 2018. Comparison of Fetal Bovine Serum and PLTMax® for Long Term Maintenance of Human Induced Pluripotent Stem Cell Derived Retinal Pigment Epithelial Cells. ISSCR Annual Meeting - Australia.
- HPL Product Information Sheet (English) | (Polish)
- PLTGold SDS
- PLTGold, Pathogen-Inactivated SDS
Bhat, S., et al. 2021. Expansion and characterization of bone marrow derived human mesenchymal stromal cells in serum-free conditions. https://doi.org/10.1038/s41598-021-83088-1
Ankrum, JA., et al. 2019. Nature vs. Nurture: Defining the Effects of Mesenchymal Stromal Cell Isolation and Culture Conditions on Resiliency to Palmitate Challenge. Front Immunol. PMID: 31134100
Alonso-Camino, V., et al. 2017. In vitro expansion of mesenchymal stem cells using media supplemented with unfractionated, heparin-free platelet lysate. Volume 19, Issue 5, Supplement, Pages e16-e17.
Burnouf, T., et al. 2016. Human platelet lysate: Replacing fetal bovine serum as a gold standard for human cell propagation. Biomaterials. 2016 Jan;76:371-87. doi: 10.1016/j.biomaterials.2015.10.065. Epub 2015 Oct 28. PMID: 26561934.
Crespo-Diaz, R.et al. 2010. Platelet lysate consisting of a natural repair proteome supports human mesenchymal stem cell proliferation and chromosomal stability. Cell Transplant. 2011;20(6):797-811. doi: 10.3727/096368910X543376. PMID: 21092406
How much human platelet lysate should I add to my media or cultures?
Cells grown in human platelet lysate typically use half the concentration compared to cells cultured in fetal bovine serum. Standard protocols suggest adding 5% concentration. However, we recommend titrating anywhere between 2-10% in order to maximize cell growth and narrow in on the best concentration for your cultures.
Is it possible to add too much HPL to my cultures?
Yes, with HPL it is possible to add too much to your cultures. We’ve seen that HPL can slow the growth of MSCs at high concentrations.
What is the difference between research-grade and clinical-grade human platelet lysate?
Both grades are manufactured under the same conditions and follow the same protocols. The clinical-grade product includes additional endotoxin testing and batch record documentation. All products have a CofA available.
What is PLTGold human platelet lysate?
PLTGold is a completely xeno-free human platelet lysate which contains all essential growth factors for healthy cell growth. Donor units are pooled in large batches and manufactured by Mill Creek Life Sciences under a proprietary method to produce highly consistent and reliable lots.
Can PLTGold be used in clinical trials?
Yes, PLTGold is a completely xeno-free supplement that does not require heparin (or used in the manufacturing process). PLTGold has an FDA registered drug master file available for reference.
What is the intended use for platelet lysate?
Large-scale expansion of cells including MSCs, T-cells, hematopoietic stem cells, iPSCs, tumor cells, and fibroblasts.
Superior xeno-free alternative to FBS and serum-free media solutions. Minimizes lot-to-lot variability.
Rapid Cell Growth
Contains growth factors that maximize cell growth without the need to worry about animal-based supplements.
Full Donor Screening
Certificates of Analysis, Certificate of Origin and Compliance available.
FDA DMF Available
HPL has been used to grow cells in various clinical trials around the world.