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Improve cell performance with a new chemically defined biomatrix for pluripotent stem cell cultures
myMATRIX iPSC is a new, xeno-free, chemically defined cell adhesion-promoting microenvironment specially formulated for the expansion of iPSCs. Offered as pre-coated cultureware, myMATRIX synthetic coatings recapitulate the major functions of the natural ECM environment, making cell culture easy, robust, and biologically relevant. myMATRIX iPSC is an effective alternative to Corning® Matrigel® and can be used with various cell culture media.
myMATRIX coatings are ideal for researchers looking for a reliable, modular biomatrix for cell therapy research and cellular agriculture applications where high performing cells are the result, not the challenge.
denovoMATRIX technologies are designed to enable the functional recreation of any extracellular matrix (ECM) based on any cell type or application in vitro by providing modular, biomimetic coating technology on your choice of cultureware. myMATRIX iPSC is available as pre-coated plates and flasks, bulk inquiries and customization is also available to accommodate your choice of coating and plasticware.
- Chemically-defined and animal component-free
- Ready-to-use and easily adaptable
- Reliable performance over animal derived substrates
- Mimics adhesion proteins such as vitronectin, laminin, and collagen
- High and consistent expression of stemness markers
Figure 1. Representative morphology images of hiPSC culture on myMATRIX iPSC. hiPSC lines grown on myMATRIX iPSC were imaged after every 5 passages in culture using phase contrast imaging. The cells show an iPSC-characteristic colony morphology with compacted individual cells that exhibit a high nuclear-to-cytoplasmic ratio. Scale bar: 100 µm.
Figure 2. Growth speed of hiPSCs during long-term culture. Human iPSCs were cultured on myMATRIX iPSC and Matrigel® for 20 passages. Growth speed was calculated as a ratio of time until confluency was reached divided by the corresponding splitting ratio. The data is displayed separately for A) P1-10 and B) P11-20 as cells were frozen at P10. Mean±standard deviation is shown.
Figure 3. Karyotypic analysis. Cytogenetic analysis using G-banding at a band level resolution below 400 revealed a 46, XY or 46, XX normal male/female karyotype. No consistent numerical or structural abnormalities were observed. Karyotype and band resolution according to ISCN 2016. Shown are representative karyotypes of three cell lines after long-term culture on myMATRIX iPSC.
|Intended use||hPSCs, iPSCs, stem cell and cell therapy research, cellular agriculture applications|
|Catalog Number(s)||C0505 (6-well plate)|
C0105 (96-well plate)
|Biological Testing||All material is tested, qualified and shown to be nontoxic according to standards ISO 10993-12 and ISO10993-5|
|Sterility||Products meet a minimum Sterility Assurance Level (SAL) according to standard ISO 11137-2 and ISO /TS 13004|
|Shelf-Life||12 months from date of manufacture|
|Storage||Store at Room Temperature. Please refer to the User Guide for additional handling instructions.|
|Quality Statement||Product does not contain materials of animal origin.|
This product is for Research Use Only and is not intended for therapeutic or diagnostic use.
- myMATRIX iPSC Product Information Sheet
- myMATRIX iPSC User Guide
- White Paper: myMATRIX iPSC – Xeno-free and chemically defined surface for high-quality pluripotent stem cell culture
- myMATRIX iPSC MSDS
References and Publications
- Kristina Thamm, K. et al. 2020. A Novel Synthetic, Xeno‐Free Biomimetic Surface for Serum‐Free Expansion of Human Mesenchymal Stromal Cells. Advanced Biosystems.
- Hamann, A., et al. 2020. Screening a chemically defined extracellular matrix mimetic substrate library to identify substrates that enhance substrate-mediated transfection. Exp Biol Med (Maywood).
- Tondera, C., et al. 2019. Highly Conductive, Stretchable, and Cell‐Adhesive Hydrogel by Nanoclay Doping. Small.
How is the denovoMATRIX surface coating produced?
The coating is based on the proprietary technology of denovoMATRIX. It is a mixture of a sulfated polysaccharide and a biomimetic peptide conjugate, which mimics functional features of the cellular microenvironment.
What is the advantage of not using proteins?
Extracellular matrix proteins are commonly used to recreate important aspects of the cellular microenvironment. However, protein-based coatings are unstable and can show compositional inconsistencies between lots. They also require pre-coating steps before each use, which can result in coating variations due to different user handling.
denovoMATRIX coatings are chemically defined with high batch consistency. They are long-term stable and are delivered ready-to-use in standard cell cultureware formats.
Does myMATRIX iPSC support a xeno-free culture of stem cells?
myMATRIX iPSC is chemically defined and free of any human or animal components. Hence, it enables the expansion of hiPSC in xeno-free culture conditions using commercially available xeno-free media.
Does myMATRIX iPSC support the long-term cultivation of hiPSC?
Yes, myMATRIX iPSC supports long-term expansion of hiPSC for up to 20 passages (over 100 days).
What is the recommended splitting method for myMATRIX iPSC?
myMATRIX iPSC supports clump-splitting as well as single-cell splitting routines, with and without supplements like a ROCK inhibitor.
Can I culture cells in any unused wells of a myMATRIX iPSC plate after performing an experiment?
It is generally recommended to use unopened myMATRIX iPSC cultureware for new experiments. However, if not all wells of the cell culture plate are used, fill unused wells with a basal medium (e.g., DMEM/F12, Advanced DMEM) and leave the medium until use.