Hanks’ Balanced Salt Solution (HBSS)
Maintaining balance in your cell cultures
Hanks’ Balanced Salt Solution (HBSS) is a buffer comprised of inorganic salts and supplemented with glucose as an energy source. It is commonly used in a variety of cell applications such as washing, diluting, and transporting cell or tissue samples. HBSS provides a buffering system designed to uphold relevant physiological pH and osmotic balance for cells maintained in non-CO2 atmospheric conditions as well as provide your cells with water and essential inorganic ions.
|Intended use||Cell and tissue washing, maintaining cells in a viable state including the collection and transport of biological specimens|
|Osmolality||275 - 295 mOsm/kg|
|Endotoxin||< 1.0 EU/mL|
|Shelf-Life||12 months from date of manufacture|
|pH||7.25 – 7.45|
|Storage||2 - 8°C|
What is Hank's balanced salt solution?
Hanks' Balanced Salt Solution (HBSS) is composed of inorganic salts and most commonly include sodium, potassium, calcium, magnesium, and chloride. The solution is buffered with phosphate and maintains a physiological pH and osmotic pressure.
What is HBSS used for in cell culture?
Hanks' Balanced Salt Solution (HBSS) is used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents. Formulations with calcium and magnesium are generally used as transport media or for reagent preparation.
What is the difference between HBSS and PBS?
As isotonic solutions, both PBS and HBSS maintain the flow of water from inside and outside the same cell or tissue. PBS is a buffered solution, while HBSS is a balanced salt solution. Both have different ingredients and forms. PBS is mostly found in liquid form, while HBSS can come in powder or liquid form.
Do you offer custom and/or bulk manufacturing of HBSS?
Yes, our standard off-the-shelf product includes the 500 mL and 1000 mL bottle sizes. We can work with you on custom formulations and bulk manufacturing.
Made in the USA
Salt Solutions are manufactured in the United States.
High Quality Buffer
Formulations are prepared using the highest quality raw materials and manufactured in ISO 13485 certified facilities.
Reliable, lot-to-lot consistency and 100% customer-focused service guaranteed.
Available in various formats and pack sizes to meet your specific research needs.