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DMEM/F12 Medium

Dulbecco’s Modified Eagle Medium/Ham’s Nutrient Mixture F12 (DMEM/F12) with L-glutamine.
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DMEM/F12 Medium is a 1:1 mixture of DMEM and Hams F-12. The combination of DMEM’s high concentration of amino acids, vitamins, and glucose, enriched with Ham's F-12 Nutrient Mixture, was found to support the growth of a wide range of mammalian cells. DMEM/F12 is a basal cell culture media that requires supplementation with serum like FBS, growth factors, or human platelet lysate.

Cells cultured in DMEM/F12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts.

This DMEM/F12 is modified as follows:
  • L-Glutamine
  • Phenol Red
Intended useCell culture, mammalian cells, stem cells
Catalog Number(s)DF1201, DF1201CS
Volume500 mL
Phenol Red IndicatorPhenol Red
Storage2°C to 8°C (protect from light)
Shelf-Life12 months from date of manufacture
Shipping ConditionsAmbient
Quality StatementThis product is for Research Use Only and is not intended for therapeutic or diagnostic use.
References and Publications
  1. Morton, H.J. 1970. A survey of commercially available tissue culture media. In Vitro 6(2):89.
  2. Ham, R.G. 1965. Clonal growth of mammalian cells in a chemically defined synthetic medium. Proc. Natl. Acad. Sci., 53:288.
  3. Smith, J.D., et al., 1960. The nucleic acid of polyoma virus. Virology 12:185.
  4. Dulbecco, R., et al. 1959. Plaque formation by the polyoma virus. Virology 8:396.
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Made in the USA

All cell culture media are manufactured in the United States.

High Quality Media

Formulations are prepared using the highest quality raw materials and manufactured in ISO 13485 certified facilities.

Reliable Formulation

Reliable, lot-to-lot consistency and 100% customer-focused service guaranteed.


Available in various formats and pack sizes to meet your specific research needs.

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