DMEM/F12 Medium is a 1:1 mixture of DMEM and Hams F-12. The combination of DMEM’s high concentration of amino acids, vitamins, and glucose, enriched with Ham's F-12 Nutrient Mixture, was found to support the growth of a wide range of mammalian cells. DMEM/F12 is a basal cell culture media that requires supplementation with serum like FBS, growth factors, or human platelet lysate.
Cells cultured in DMEM/F12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts.
This DMEM/F12 is modified as follows:With
- Phenol Red
|Intended use||Cell culture, mammalian cells, stem cells|
|Catalog Number(s)||DF1201, DF1201CS|
|HEPES Buffer||No HEPES|
|Phenol Red Indicator||Phenol Red|
|Storage||2°C to 8°C (protect from light)|
|Shelf-Life||12 months from date of manufacture|
|Quality Statement||This product is for Research Use Only and is not intended for therapeutic or diagnostic use.|
References and Publications
- Morton, H.J. 1970. A survey of commercially available tissue culture media. In Vitro 6(2):89.
- Ham, R.G. 1965. Clonal growth of mammalian cells in a chemically defined synthetic medium. Proc. Natl. Acad. Sci., 53:288.
- Smith, J.D., et al., 1960. The nucleic acid of polyoma virus. Virology 12:185.
- Dulbecco, R., et al. 1959. Plaque formation by the polyoma virus. Virology 8:396.
Made in the USA
All cell culture media are manufactured in the United States.
High Quality Media
Formulations are prepared using the highest quality raw materials and manufactured in ISO 13485 certified facilities.
Reliable, lot-to-lot consistency and 100% customer-focused service guaranteed.
Available in various formats and pack sizes to meet your specific research needs.