Low Protein Media, BSA-Free

Low Protein Media, BSA-Free

DMEM/F12 Medium, HEPES

DMEM/F12, with HEPES, and L-Glutamine is available in a 500 mL bottles and has been optimized for stem cell cultures including hESC and hiPSC applications.

Custom bulk manufacturing and pack sizes also available. Contact our Customer Service team by emailing orders@captivatebio.com for more information.
As low as $45.00
In stock

DMEM/F12 Medium is a 1:1 mixture of DMEM and Hams F-12. The combination of DMEM’s high concentration of amino acids, vitamins, and glucose, enriched with Ham's F-12 Nutrient Mixture, was found to support the growth of a wide range of mammalian cells. DMEM/F12 is a basal cell culture media that requires supplementation with serum like FBS, growth factors, or human platelet lysate.

Cells cultured in DMEM/F12 with HEPES include mouse embryonic stem cell, epithelial cells, neural stem cells, human embryonic stem cells, mesenchymal stem cells, induced pluripotent stem cells, and hematopoietic stem cells.

This DMEM/F12 is modified as follows:
  • L-Glutamine
  • Phenol Red
Intended useCell culture, mammalian cells, stem cells, hPSCs, mouse embryonic stem cells, hiPSCs
Catalog Number(s)DFHG01 (500 mL), DFHG02 (1000 mL)
Volume500 mL and 1000 mL
HEPES Bufferwith HEPES
Phenol Red IndicatorNo Phenol Red
Storage2°C to 8°C (protect from light)
Shelf-Life12 months from date of manufacture
Shipping ConditionsAmbient
Quality StatementThis product is for Research Use Only and is not intended for therapeutic or diagnostic use.
References and Publications
  1. Morton, H.J. 1970. A survey of commercially available tissue culture media. In Vitro 6(2):89.
  2. Ham, R.G. 1965. Clonal growth of mammalian cells in a chemically defined synthetic medium. Proc. Natl. Acad. Sci., 53:288.
  3. Smith, J.D., et al., 1960. The nucleic acid of polyoma virus. Virology 12:185.
  4. Dulbecco, R., et al. 1959. Plaque formation by the polyoma virus. Virology 8:396.
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Made in the USA

All cell culture media are manufactured in the United States.

High Quality Media

Formulations are prepared using the highest quality raw materials and manufactured in ISO 13485 certified facilities.

Reliable Formulation

Reliable, lot-to-lot consistency and 100% customer-focused service guaranteed.


Available in various formats and pack sizes to meet your specific research needs.

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