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DMEM/F12 Medium, HEPES

DMEM/F12 Media, with HEPES and L-Glutamine is a basal formulation used for supporting the growth of various stem cells including human ESCs and hiPSCs.
Grouped product items
SKUSIZEPRICE QTY
DFHG01500 mL
$45.00
- +
DFHG021000 mL
$75.00
- +
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Reviews
Description
Specifications
More Information
SKUDFHG-CT
Size1000 mL
Intended useCell culture, mammalian cells, stem cells, hPSCs, human ESCs, mouse embryonic stem cells, hiPSCs
Catalog Number(s)DFHG01 (500 mL), DFHG02 (1000 mL)
Volume500 mL and 1000 mL
FormLiquid
HEPES Bufferwith HEPES
GlutamineL-Glutamine
Phenol Red IndicatorNo Phenol Red
Storage2°C to 8°C (protect from light)
Shelf-Life12 months from date of manufacture
Shipping ConditionsAmbient
Quality StatementThis product is for Research Use Only and is not intended for therapeutic or diagnostic use.
ManufacturerCaptivate Bio

DMEM/F12 Medium is a 1:1 mixture of DMEM and Hams F-12. The combination of DMEM’s high concentration of amino acids, vitamins, and glucose, enriched with Ham's F-12 Nutrient Mixture, was found to support the growth of a wide range of mammalian cells. DMEM/F12 is a basal cell culture media that requires supplementation with serum like FBS, growth factors, or human platelet lysate.

Cells cultured in DMEM/F12 with HEPES include mouse embryonic stem cell, epithelial cells, neural stem cells, human embryonic stem cells, mesenchymal stem cells, induced pluripotent stem cells, and hematopoietic stem cells.

This DMEM/F12 is modified as follows:
With
  • L-Glutamine
  • HEPES
Without
  • Phenol Red

Standard packaging includes 500 mL and 1000 mL bottles. Other volumes and bioprocessing bags are available upon request. To inquire about bulk or custom options, please inquire here

Resources
Documents
References and Publications
  1. Morton, H.J. 1970. A survey of commercially available tissue culture media. In Vitro 6(2):89.
  2. Ham, R.G. 1965. Clonal growth of mammalian cells in a chemically defined synthetic medium. Proc. Natl. Acad. Sci., 53:288.
  3. Smith, J.D., et al., 1960. The nucleic acid of polyoma virus. Virology 12:185.
  4. Dulbecco, R., et al. 1959. Plaque formation by the polyoma virus. Virology 8:396.
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