Y27632 (2HCl) Small Molecule

Y27632 (2HCl)

CHIR99021

Rating:
100% of 100
CHIR99201 potentiates self-renewal of human and mouse embryonic stem cells and enhances reprogramming of somatic cells into stem cells. Powder, 10 mg
As low as $125.00
In stock
SKU
SML01B
Small Molecules for Stem Cell Research

CHIR99021 is a highly-potent and -selective inhibitor of GSK3α (IC50 of 10) and GSK3β (IC50 of 6.7), exhibiting over 500-fold selectivity when compared to its nearest homologs. CHIR99021 has been an effective component within differentiation protocols of pluripotent stem cells (PSCs) into human pancreatic beta cells (Pagliuca, et al.), neural progenitor cells (Li, et al. & Qi, et al.), cardiovascular progenitor cells (Cao, et al.), and functional cardiomyocytes (Lian, et al. & Burridge, et al.).

Additionally, CHIR99021 has been shown to promote the induction of human PSCs (Li, W. et al.) and self-renewal of PSCS for maintenance purposes (Ying, et al.).

Description: Highly selective GSK-3 inhibitor; acts as Wnt activator
Alternative Names: CHIR99021, CT99021
Chemical Name: 6-[[2-[[4-(2,4-Dichlorophenyl)-5-(5-methyl-1H-imidazol-2-yl)-2 pyrimidinyl]amino]ethyl]amino]-3-pyridinecarbonitrile
Purity: ≥ 98%

Specifications
Molecular NameCHIR99021
FormPowder
Alternative NamesCT 99021, CHIR-99021, CHIR 99021
Chemical Name6-[2-[4-(2,4-Dichlorophenyl)-5-(4-methyl-1H-imidazol-2-yl)pyrimidin-2- ylamino]ethylamino]pyridine-3-carbonitrile
Chemical FormulaC22H18Cl2N8
Molecular Weight465.34
CAS Number252917-06-9
PurityGreater than 95% by LCMS analysis
Physical AppearanceWhite to yellow (Solid)
TargetGSK3; Autophagy
Shelf-Life≥ 2 years (powder)
ShippingRoom Temperature
StorageStore at -20°C
Quality StatementThis product is for Research Use Only and is not intended for therapeutic or diagnostic use.
Documents
References and Publications
  1. Qi, et al. 2017. Combined small-molecule inhibition accelerations the derivation of functional cortical neurons from human pluripotent stem cells. Nature Biotechnology 35(2): 154-163.
  2. Burridge, et al. 2015. Chemically defined culture and cardiomyocyte differentiation of human pluripotent stem cells. Curr Protoc Hum Genet. 87(1): 1-15.
  3. Pagliuca, et al. 2014. Generation of functional human pancreatic β cells in vitro. Cell 159: 428-439.
  4. Cao, et al. 2013. Highly efficient induction and long-term maintenance of multipotent cardiovascular progenitors from human pluripotent stem cells under defined conditions. 23:1119-11132.
  5. Lian, et al. 2013. Directed cardiomyocyte differentiation from human pluripotent stem cells by modulating Wnt/β-catenin signaling under fully defined conditions. Nature Protocols 8(1): 162-175.
  6. Li, et al. 2011. Rapid induction and long-term self-renewal of primitive neural precursors from human embryonic stem cells by small molecule inhibitors. PNAS. 108(20): 8299-304. Li, W., et al. 2009. Generation of human-induced pluripotent stem cells in the absence of exogenous Sox2. Stem Cells 27: 2992-3000.
  7. Ying, Q., et al. 2008. The ground state of embryonic stem cell self-renewal. Nature 453: 519-523.
Write Your Own Review
Only registered users can write reviews. Please Sign in or create an account
We found other products you might like!