BIO-HEMATO™ Karyotyping Medium
Cytogenetic analysis of human hematopoietic cells using bone marrow aspirates is a standard practice in hematology. Fresh cells or cells grown in short-term cultures often yield an insufficient number of mitotic cells and repeated aspirations are required. BIO-HEMATO™ Karyotyping Medium is a ready-to-use medium optimized to stimulate the proliferation of human hematopoietic cells from bone marrow as well as peripheral blood.
This medium is particularly effective for karyotyping of acute non-lymphocytic leukemias and various stages of chronic myelogenous leukemia as well as other hematological disorders such as myelodysplastic syndrome and polycythemia vera.
BIO-HEMATO™ Karyotyping Medium is based on MEM-alpha basal medium supplemented with L-Glutamine, FBS, antibiotics (gentamicin) and conditioned medium. BIO-HEMATO™ Karyotyping Medium is supplied as frozen medium, which is ready for use after thawing.
Packaged in single bottles, ready-to-use with no mixing required. For in vitro diagnostic use.
- Manufactured in compliance with cGMP guidelines
- Fast growth and high mitotic index
- Complete, ready-to-use media
- Quality control tested for sterility, pH, osmolality and endotoxin
Culture of bone marrow and peripheral blood cells for chromosome analysis
The hematopoietic cell karyotyping method was developed to provide information about chromosomal abnormalities. In the presence of a conditioned medium, acute and chronic nonlymphocytic leukemic cells in bone marrow and peripheral blood cultures are stimulated to enter into mitosis by DNA replication. After 48-72 hours, a mitotic inhibitor is added to the culture to stop mitosis in the metaphase stage. After treatment by hypotonic solution, fixation and staining, chromosomes can be microscopically observed and evaluated for abnormalities.
|Intended use||Proliferation of human hematopoietic cells from bone marrow and peripheral blood|
|Catalog Number(s)||01-200-1A (500 mL) |
01-200-1B (100 mL)
|Storage||Store at - 20° C After thawing, the medium should be stored at 2-8° C. The medium should be used within 10 days after thawing. Protect the medium from light.|
|Quality Statement||For in vitro diagnostic use. The medium is not intended for therapeutic use. Listed in Europe under CE IVD class I, thus complying with European In-Vitro Diagnostic Devices Directive (98/79/EC) requirements.|
|Testing||Quality control tested for sterility, pH, osmolality and endotoxin|
|Manufacturer||Biological Industries Israel Beit Haemek Ltd.|