BIO-PB™ Karyotyping Medium
Blood cell karyotyping is an important tool in modern human cytogenetics, providing information about chromosomal abnormalities, their frequency in the population, and the relationship between specific abnormalities and phenotypic effects. Human cytogenetic studies involve the examination of a stimulated lymphocyte after blocking cell division at metaphase with an inhibitor of spindle formation. The nuclear membrane breaks down and chromosome condensation takes place as usual, but the chromosomes fail to organize themselves into a metaphase plate. This gives an appearance quite unlike a natural metaphase, in that the chromosomes are free within the cytoplasm. Subsequent processing and staining allows clear visualization of the chromosomes. The chromosomes can be stained either by a technique that gives a fairly uniform intensity, or by a technique that gives differential staining along the length of the chromosome.
BIO-PB™ Karyotyping medium, with PHA-M (Phytohemagglutinin-M) is specifically optimized for short-term culture of peripheral blood lymphocytes for chromosome analysis. This formulation consists of a RPMI medium supplemented with serum, L-glutamine, antibiotics (gentamicin) and PHA-M. The PHA-M potency for mitotic stimulation is measured for optimized concentration.
BIO-PB™ Karyotyping Medium is supplied as frozen medium, which is ready for use after thawing.
|Intended use||Short-term culture of peripheral blood lymphocytes for chromosome analysis|
|Storage||Store at - 20° C|
|Stability||After thawing, store at 2-8° C. Medium should be used within 10 days after thawing. Protect from light.|
|Quality Statement||For in vitro diagnostic use. The medium is not intended for therapeutic use. Do not use if a visible precipitate is observed in the medium.|
|Testing||Quality control tested for sterility, pH, osmolality, and endotoxin. In addition, each batch is tested for performance using primary human peripheral blood lymphocytes cultured for 72 hours.|
|Manufacturer||Biological Industries Israel Beit Haemek Ltd.|
References and Publications
- Moorhead, P.S., et al, Chromosome Preparations of Leukocytes Cultured from Human Peripheral Blood, Exp. Cell. Res., 20:613-616 (1960)
- Nowell, P.C., Phytohemagglutinin - An Initiator of Mitosis in Cultures of Normal Human Leukocytes, Cancer Res., 290:462-466 (1960)
- Barch, M.J. (ed), The Association of Cytogenetic Technologists Laboratory Manual, Second Edition (1991)